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General Information

In this workshop, you will be learning how to analyse RNA-seq count data, using R. This will include reading the data into R, quality control and performing differential expression analysis and gene set testing, with a focus on the limma-voom analysis workflow. You will learn how to generate common plots for analysis and visualisation of gene expression data, such as boxplots and heatmaps. This workshop is aimed at biologists interested in learning how to perform differential expression analysis of RNA-seq data when reference genomes are available.

Who: The course is aimed at graduate students and other researchers. Some basic R knowledge is assumed. This is not an introduction to R course. If you are not familiar with the R statistical programming language we strongly encourage you to work through an introductory R course before you attend this workshop. We recommend the Software Carpentry R for Reproducible Scientific Analysis lessons up to and including vectorisation (topic 9).

Where: Seminar Room 2, WEHI (1G Royal Parade, Parkville VIC 3051, Australia). Get directions with OpenStreetMap or Google Maps.

Requirements: Participants must bring a laptop with a Mac, Linux, or Windows operating system (not a tablet, Chromebook, etc.) that they have administrative privileges on. They should have a few specific software packages installed (listed below).

Contact: Please mail combine@combine.org.au for more information.

Registration: register here. Registration is $56/$62 for ABACBS members, and $80/$89 for non-members (for students/non-students respectively).

Schedule

Note: this is a preliminary schedule. There may be changes to the timing and content.

If you have any trouble installing the software or packages, please arrive at 9am on the first day so we can help before the workshop starts.

Etherpad: https://pad.carpentries.org/2018-09-26-RNAseq-Melbourne.
We will use this Etherpad for chatting, taking notes, and sharing URLs and bits of code.

Syllabus

Data

Please download these data files and save them to your laptop before the workshop:

• Mouse mammary data (counts): https://figshare.com/s/1d788fd384d33e913a2a

Setup

To participate in this workshop, you will need access to the software described below. In addition, you will need an up-to-date web browser.

Even if you already have R installed, it is important that you have the latest version because some of the packages we will be using will not work with earlier versions of R. The latest version (available through the links below) is 3.5.1. For Mac OS X (10.6 and above) the latest version is 3.5.1 (available from here) and requires Clang 6.0.0 and GFortran (both available from here).

Software Carpentry maintains a list of common issues that occur during installation as a reference for instructors that may be useful on the Configuration Problems and Solutions wiki page.